Journal: bioRxiv
Article Title: Chronic but not acute morphine exposure reversibly impairs spike generation and repetitive firing in a functionally distinct subpopulation of orexin neurons
doi: 10.1101/2025.03.20.644444
Figure Lengend Snippet: A. Schematic of experimental cohorts, injection timing and tissue preparation. B. Low power (4x objective) and C. higher power (40x objective) epifluorescence images of EGFP-positive neurons within the orexin neuron field. Anti-orexin-A immunofluorescence (red), anti-GFP immunofluorescence (green). D. Efficacy (percent orexin immunopositive neurons also expressing GFP immunofluorescence) and specificity (percent GFP immunopositive neurons also expressing orexin immunofluorescence). E ., F . Brightfield low power (E) and higher power (F) of GFP-immunopositive neurons visualized with VIP. Yellow polygon delineates the soma outline (F) used for measurements in G-J. G . GFP-immunopositive soma area per mouse from saline-treated (S; blue symbols) and morphine-treated (M; red symbols) mice. H. Histogram of all measured GFP-immunopositive soma sizes from each treatment group (S, blue, n = 356 cells; M, red, n = 440 cells). I . Cumulative fraction of soma sizes from each treatment group (S, blue symbols; M, red symbols; Kolmogorov-Smirnov test p < 0.0001. Abbreviations: f, fornix; S, saline-treated; M, morphine-treated. Numbers are reported as mean ± sem.
Article Snippet: For identification of GFP, sections were incubated with sheep polyclonal anti-GFP antibody (1:1,000, Novus Biologicals, #NB100-62622, lot#1710).
Techniques: Injection, Immunofluorescence, Expressing, Saline
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